The RMT union confirmed on Monday that two 24-hour strikes have been planned on the Metropolitan line.
The first will take place from 11.30am on January 20 to 11.29am on January 21.
While the second is expected to occur from 11.30am on February 10 to 11.29am on February 11.
The union said the latest decision follows an "overwhelming" vote for action by drivers on the Metropolitan line based at the Neasden Depot.
RMT general secretary Mick Lynch said: "There’s a toxic culture developing at London Underground which amounts to the wholesale ripping up of normal procedures and agreements and our members at Neasden have said loud and clear that enough is enough.
"I am calling on tube bosses to get a grip and stop this wholesale undermining of normal industrial relations."
A Transport for London (TfL) spokesperson said: “We’re disappointed that the RMT has confirmed strike action at Neasden Depot and will be talking with them this week to resolve the matter.”
Night Tube strikes
RMT has also said that weekend strikes on the Night Tube will continue through to the summer.
The union says that TfL should hire additional drivers to cover overnight shifts on the weekend, while the transport body has said that they will only have to work four night-shift weekends per year, and can swap shifts.
Andy Lord, managing director of London Underground, said: “We’re disappointed that, once again, the RMT is continuing to push for strike action that is likely to cause further unnecessary disruption.
“We’re calling on the RMT to join us for talks so we can work together to resolve this dispute around roster changes, which mean no job losses and greater flexibility for drivers.
“If these six months of action do go ahead, we will continue to operate as regular a service as possible.
“However, customers are advised to check before they travel and use buses to complete their journeys where required.
“I apologise to them for the impact this unnecessary action will have on their journeys.”