Transport for London (TfL) said services on the busiest Central and Victoria lines will run throughout the night on Fridays and Saturdays from Saturday 27 November.
Over 130,000 people signed a petition calling for the Night Tube to be reinstated to help protect women travelling at night.
The petition was set up following the recent murders of Sabina Nessa and Sarah Everard, whose deaths have sparked debates around women’s safety in London.
The Mayor of London, Sadiq Khan, said: “The reason why I was so keen to be the first Mayor in London’s history to start the Night Tube in 2016 and to restart it now, after the Covid pause, is because I know how important this is to London’s thriving night-time economy, to London’s recovery and to the confidence and safety of everyone travelling home at night, particularly women and girls.
“ I am determined to make our city as safe as possible for all Londoners.
“That’s why I’m delighted to see the return of the Victoria and Central Night Tube lines next month, which will make a huge difference to people travelling around our city at night and making their way home, offering them an additional safe, reliable transport option.
“I will continue to work with TfL and the Government to do everything we can to bring back the full Night Tube network as soon as possible.”
TfL said that the Night Tube was suspended during the pandemic because drivers were needed for the Tube service during the day, where demand has been higher.
London Underground was also impacted by staff absences due to the major disruption caused by the pandemic at a time when Tube ridership dropped by over 90 per cent.
Nick Dent, London Underground’s Director of Customer Operations, said: “The return of the Night Tube will be a huge boost to the night-time economy and help our customers travel around London at weekends.
“The Tube has played a vital role during the pandemic and will continue to be vital to London’s recovery.
“We continue to run a regular, orderly and clean service and will continue to work hard to restore the full Night Tube network as soon as possible.”
Michael Kill, Chief Executive Officer, Night Time Industries Association said: "The Night Tube’s return across the Central and Victoria line from the end of November is a welcome addition to London’s Night Time Economy infrastructure, and a step in the right direction.
“With a focus on vulnerability, and the safety of women at night, and thousands of night workers within London we must push to get the full service established at the earliest opportunity and open London’s late night economy fully to the public."
TfL have said that they are also working on getting the Jubilee, Northern and Piccadilly Night Tube services up and running again too.