The RMT Union has announced that 4,000 of its members will stage a network wide walkout in protest of what it claims are Transport for London’s (TfL) plans to cut 600 jobs to reduce costs.
The action will start a minute after midnight and last for 24 hours and will impact all London Underground lines.
The union also announced an overtime ban from next Friday, June 3, the second day of the celebrations, until July 10.
This is a separate walkout to the one of June 3, in which station staff at Green Park and Euston will go on strike, amid claims of a toxic work environment created by a "bullying manager".
TfL has insisted that no jobs will be lost.
Andy Lord, TfL’s chief operating officer, said on Monday: “We are extremely disappointed that the RMT has announced unnecessary strike action on June 6 and share Londoners’ frustrations that this, and the linked action short of strike, has been designed to disrupt the Jubilee weekend.
“It is particularly surprising that the RMT has threatened to spoil this moment when the nation is coming together as nobody has or will lose their jobs as a result of the proposals we have set out and there have been no proposals on pension changes.
“If the RMT chooses to go ahead with this unnecessary action, we will do everything we can to minimise any disruption and ensure everyone can still make the most of the capital throughout the Jubilee weekend.
“The devastating impact of the pandemic on TfL finances has made a programme of change urgently necessary but this change is being delivered in a way that ensures nobody loses their jobs so we’re calling on the RMT to work with us, rather than disrupting London’s recovery and our customers, who deserve better.
“We’ve been in regular talks with the RMT and are hoping to find an urgent resolution to this dispute to ensure they can call off this unnecessary action.”
However RMT general secretary Mick Lynch said: "TfL is trying to bulldoze through 600 job losses on London Underground and our members are not prepared to accept that.
"Station staff play a crucial role in serving the travelling public and were heroes during the 7/7 terrorist attacks.
"Instead of seeking to cut jobs, TfL and mayor Sadiq Khan need to put further pressure on the government to secure increased funding for the network so we can have a properly staffed modern 21st century Tube."